How Rapid Covid-19 PCR Tests Work

rapid pcr test

Due to the continuous rise in Covid-19 cases around the world, effective and accurate methods of making diagnoses are necessary. An effective Covid-19 diagnosis test should be quick and accurate at the same time. The rapid Covid-19 PCR test detects the virus’ genetic material (RNA) through a lab technique known as polymerase chain reaction (PCR).

Unlike the diagnostic tests for Covid-19 antibodies that are recommended for people who have recovered from an infection suspected to be Covid-19, the polymerase chain reaction (PCR) test is diagnostic testing for people who are currently infected by Covid-19. The tests directly detect the presence of antigens rather than the body’s immune response. The PCR test is done in the lab to identify even very small amounts of the virus. This is done by detecting and amplifying the virus’s genetic material (RNA) to detectable levels. By detecting the virus’s RNA, which shows up in the body before antibodies (body’s immunity) or signs and symptoms of the disease are present, the test can tell whether someone has the virus in the early stages. PCR gives an accurate indication of infected people. This is one of the main advantages of PCR diagnostic tests. The transmission chain can be easily broken, and we can get a clear picture of what is happening. The test can be used for all people, including those who are pre-symptomatic, asymptomatic, and those who were in contact with a person with Covid-19.

PCR test uses a sample from the person with associated signs and symptoms. Samples are extracted through several methods, such as using a swab, collecting coughed up material (sputum), throat swabs, or deep airway material obtained using a catheter (probe) or saliva (nasopharyngeal). The accuracy of the test can vary depending on the method used to obtain the sample. From a diagnostic point of view, this method of obtaining the sample may have very low levels of the virus that may fail to be detected depending on the time passed since infection. Nevertheless, in most cases, the sample is obtained using a swab inserted into the nose or throat. This method has been proven to contain a detectable amount of the virus using the most available equipment. Other methods are also used depending on the patient, how fast the results are needed, among other factors. PCR tests usually take about 3-5 days to give results if the analysis is done in a lab or performed in a clinical setting. Health care settings can also benefit from this rapid PCR test that doesn’t require laboratory analysis and the time to transport samples to the said lab. There are options for our device to run 96 individual samples tested in 30 minutes time. The results can be shared via a report on the included free mobile app. Hospital groups in countries around the world could benefit from this rapid PCR solution to help in triage.

The US Food and Drug Administration (FDA) recently approved various at-home rapid Covid-19 molecular test kits through an Emergency Use Authorization (EUA). In contrast to the previous kits, which required the user to send samples back to the lab for analysis, these provide results within a very short time (about 30 minutes) and have easy-to-understand instruction on how to use them. Similar to the Covid-19 antibody test kits, the user self-collects the sample using the provided swab and follows the instructions provided to perform the analysis. Usually, after collecting the sample, the user must swirl it within a sample vial included in the testing kit. The vial is then placed in the testing unit that warms the samples with heat and the change in color of sample and buffer is the indicator of infection or lack of infection. Since PCR is a diagnostic test for persons who currently have Covid-19, a positive result indicates that the person has been infected with Covid-19. Isolation and quarantine measures should, therefore, follow if the test is positive.

The annealing temperature (typically between 48-72°C) is related to the melting temperature (Tm) of the primers and must be determined for each primer pair used in PCR. During the extension step (typically 68-72°C) the polymerase extends the primer to form a nascent DNA strand. This process is repeated multiple times (typically 25-35 cycles), and because each new strand can also serve as a template for the primers, the region of interest is amplified exponentially. The final step of the PCR is generally a longer, single temperature step (often 5-10 min at 68-72°C) that allows for the completion of any partial copies and the clearance of all replication machinery from the nascent DNA. Once the PCR is complete, the thermal cycler is set to 4°-10°C to maintain product integrity until such time as the tubes can be removed from the machine.

Generally, if you are looking for an In vitro diagnostic (IVD) device that can quickly diagnose a COVID-19 related case, then you can bet on a rapid Covid-19 PCR test. These test devices are easy to use and they offer the greatest potential for screening asymptomatic and pre-symptomatic patients. Based on research, they have contributed a lot to the expansion of testing capabilities in the US. The diagnostics and detection of the live virus can show a negative or positive active viral result values with high sensitivity, specificity, and accuracy. While some people may have an inhibition about the sample extraction process, but it isn’t too uncomfortable compared to other methods for other medical tests. Large numbers of a sample can be collected from a population to help identify infected persons. This data can be used for emergency guidance in stopping the community infectious spread of the virus. The specific probability of spread of the virus from detected cases can be better observed and help modify current restrictions.

When we perform quick analysis of specimens with high accuracy the risk of spread is reduced greatly. Combining this testing solution with testing for COVID-19 antibodies as well as antigen tests gives a full suite of solutions. An Antibody tests kit can show if a patient is immune to future infection. Performing different types of tests helps in solidifying findings from primary initial tests that diagnosed and identified patients with coronavirus.

If you are looking to buy large quantities of FDA EUA approved rapid Covid-19 PCR tests online, then you are safe when you get them from Rapid Connect Medical. We distribute large quantities of rapid tests across the globe at affordable prices. Bulk volume orders receive special pricing.

Rapid Connect Medical distributes various rapid Covid-19 testing kits. We are among the leading distributors of accurate, rapid Coronavirus test kits. The accuracy of any rapid testing kit depends on the sensitivity and specificity. These are important factors, especially when it comes to PCR testing. An efficient kit should be able to detect very low levels of the virus at the early stages of infections. High sensitivity is required to be able to capture the low levels of the virus. In the same way, high specificity is needed to distinguish the genetic materials of the virus from a sample with other genetic materials. Our kits are verified to be highly accurate with high sensitivity and specificity. We also provide detailed but easy-to-use instruction materials that anyone can understand without any special skills.

Our reliable Covid-19 test kits provide results within a few minutes. We are committed to our fight against Covid-19, and hence we supply both small and large quantities at affordable prices. If you are looking for a place to purchase reliable Covid-19 antibody test kits and other testing kits, contact us now for more information.

Leave a Reply

Your email address will not be published. Required fields are marked *

Translate »