How Do COVID-19 Antibody Test Kits Work?

How Do COVID-19 Antibody Test Kits Work? - Rapid Connect Medical

Coronavirus testing is vital to the efforts towards tackling the spread of COVID-19 across the globe. Government laboratories and private clinics are doing mass testing to quickly help in the identification and isolation of infected individuals; a move that keeps the rest of the population safe.

Health officials are either using RT-PCR, antibody response, or both to test for SARS-CoV2 in people that are suspected to have been infected by the virus.  To undertake these tests, the professionals need test kits and reagents. Antibody response tests will require reliable coronavirus antibody test kits.

A COVID-19 antibody test kit is a qualitative membrane-based immunochromatographic assay that uses qualitative lateral flow to detect IgM and IgG antibodies to SARS-CoV-2 in human whole blood, serum, or plasma samples. A coronavirus antibody testing kit works similar to that of a pregnancy test kit only that in this case it detects antiviral antibodies instead of pregnancy hormones.

In this article, you are going to learn how a COVID-19 IgM/IgG rapid test kit works to determine whether or not an individual has been exposed to the novel coronavirus.

Covid-19 Antibody Test Kit

The one-step rapid COVID-19 antibody test kit distributed by Rapid Connect Medical contains six components: a blood pipette, a silver sterile packet, a lancet, an alcohol swab, a buffer solution, and an antibody detection test cassette.  These kits are designed for in vitro diagnostics (IVDs) use only.

An antibody detection test cassette contains antigens from SARS-CoV-2 combined with gold nanoparticles. The device has two wells (one for specimen and one buffer) and a control region on the upper end.  Two types of antibodies are of great importance in diagnostic tests for COVID-19 antibodies: Immunoglobulin M (IgM) and Immunoglobulin G (IgG).

The importance of the gold nanoparticles in the detection test cassette is to create a colored line in the control region.  Antigens targeting a specific antibody are located in their respective regions on the nitrocellulose membrane of the test cassette. The IgG and IgM regions are coated with anti-human antibodies for each.

Testing Process

During testing, the COVID-19 antigen particles in the test cassette react with the human specimen to form a mixture. The mixture then moves upwards by capillary action through the membrane chromatographically to the IgG and IgM regions where a second reaction takes place. The use of the buffer solution in the test is to prevent any change in pH value and assist the human blood specimen to flow.

If the specimen contains IgM antibodies against COVID-19, then the IgM test line region turns red. If the sample mixture contains IgG antibodies to COVID-19, then the IgM test region will display a red-colored line. All test regions will turn red when both the IgM and IgG antibodies are present in the specimen. And if no antibodies are present in the test sample, only the control line region will change color.

The change in color on the control line region does not detect a presence of antibodies, it just indicates the occurrence of membrane wicking and use of proper volumes of the specimen.  Testing using a rapid COVID-19 antibody test kit is fast. Sometimes the results become visible after two minutes, but they should be read after ten minutes.

Advantages of using Covid-19 Antibody test kits

The COVID-19 antibody or serology tests are usually performed in public health laboratories or other health professional settings. They are designed for use by a specialist or clinician rather than by individuals who wish to know their COVID-19 infection status.

Antibodies present a historic picture of past infections when they are present in the bloodstream. The fact that serology or antibody testing gives an account of past infections and the level of herd immunity in the population makes it a powerful tool. As antibodies take weeks to develop, so some tests might give false-negative results.

Rapid COVID-19 antibody test kits are relatively faster and potentially cheaper when compared to lab methods. Since they are not as accurate as RT-PCR, they are carefully designed and validated. Quality control and validation add significant cost to the design of these kits. They also rely on novel microbiology and blood testing technology to improve their accuracy. 

The advantage of diagnostic tests for COVID-19 antibodies is that you can be able to determine whether a patient has COVID-19 or has recovered from the disease. Different from RT-PCR, antibody lateral flow tests detect the patient’s immune antibody response to the virus. Lateral flow technology offers a quick solution to determine whether a patient has been infected with the COVID-19 coronavirus.


Now that you have a good idea of how the COVID-19 antibody test kits work, feel free to visit Rapid Connect Medical for more information on how to place your order. The company is an exclusive global distributor of Coronavirus (SARS-CoV-2) IgM/IgG rapid test kits offering highly accurate testing at an affordable price.

Rapid Connect Medical distributes large orders of Coronavirus (SARS-CoV-2) IgM/IgG rapid test kits to global professional sports league and arenas. Rapid Connect Medical is the leading provider of Covid-19 Antibody test kits in small quantities for individuals25 test kit packs, as well as bulk orders for large countries, companies, and organizations.  If your institute or corporation is in the need of antibody test kits, feel free to email our sales team.  The company covers the USA, Canada, South America, and European territories. Therefore, if you are from these areas, Rapid Connect Medical will be able to assist you with availability, pricing, and ordering. Our test kit is considered to be the best product on the market for rapid Covid-19 antibody testing.

The rapid COVID-19 antibody test kits you will find on the market are designed to detect SARS-CoV-2-specific antibodies. The ones distributed by Rapid Connect Medical are FDA research approved and validated by a comprehensive clinical study.  The CDC testing standards for laboratories were strictly followed during the clinical trials for our antibody test kit.  This is a serology elisa based test for SARS-CoV-2 antibodies in serum or plasma components of blood. Information on the collection data and validation of lab samples can be found here for review.

The sampling done with the rapid COVID-19 antibody test kit cassette using lateral flow from Rapid Connect Medical will give you the IgG and IgM detectable diagnostics results from between two and ten minutes. It can be used for reference in combination with the results from an RT-PCR (reverse transcriptase-polymerase chain reaction) test. This positive or negative result can be detected as soon as 3 days after human contact or exposure to COVID-19 (sometimes before the onset of symptoms occur).  If you were exposed to COVID-19, and do test positive, you should seek care from your doctor for treatment and continue to test for IgG antibodies daily until you are cleared to leave quarantine. The sensitivity, specificity, and accuracy or our antibody test kit is very high.  This testing method for the public gives real time results.

The kits used for diagnostic tests for COVID-19 antibodies are usually designed for research use or qualitative detection of coronavirus, and not home use. Also, they can only be used to test the presence of IgG and IgM antibodies against SARS-CoV-2. Since they are aiding tools to RT-PCR or CT scanning, a clinical diagnosis of COVID-19 should be done by a healthcare professional or physician.  The total number of patients fighting this severe virus around the world is staggering.  Infected people should seek medical attention from health care providers to determine the best treatment of the virus.

Our diagnostics test kits are FDA EUA (Emergency Use Authorization) Approved, have the CE Declaration for authorized European Union distribution, and have an authorization for use by the HHS for Pharmacies and Pharmacists.  Rapid Connect Medical’s COVID-19 Antibody test kit is 98.23% accurate with results in 2 to 10 minutes.  Don’t take a risk using inferior test kits, our Antibody Test kits have been tested in a biotechnology laboratory to obtain the FDA EUA Approval!  Our kits also passed the clinical tests of the National Cancer Institute and WHO.  The development of the technology for this screening standard with confirmed scientific accuracy is unique to Rapid Connect Medical‘s Antibody Test Kit.  If you are interested in order a large quantity and have questions, feel free to book a call with our sales team.  We have the capacity to support your required production needs.

We offer fast shipping methods for our antibody test kits at an affordable rate.  You can order test kits online at

Stop the Spread – Test Yourself Now

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